Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Gastroenterology. 2016 Oct 18;151(6):1206–1217. doi: 10.1053/j.gastro.2016.09.064

Figure 5.

Figure 5

Circulating IL22 is elevated in CP and associated with cigarette smoking among CP patients. (A) Serum from health control subjects (HC, n = 11) and CP patients (CP) who were non-smokers (Non, n = 18), current-smokers (Current, n = 10), or ex-smokers (Ex, n = 11) were collected and IL22 analyzed using an enzyme-linked immunosorbent assay (mean ± SEM, one-way analysis of variance, Tukey’s post-hoc test). (B) Naïve CD4+ T cells were enriched using magnetic beads from peripheral blood mononuclear cells (PBMCs) of healthy blood donors (buffy coat), and then activated with antibodies to CD3 and CD28 in the presence of AhR agonist (TCDD or BaP) with or without the AhR antagonist (CH223191) for 5 days. Flow cytometry plots show frequency of IL17α and IL22 expression in activated CD4+ T cells. Data are representative of 4 independent experiments. (C) Bar graphs show frequency of IL22+ - activated T cells, presented as mean ± SD (n = 4, unpaired 2-tailed Student t test).