Fig. 5. MGE/DGE–Amp selectively kill uropathogenic E. coli in the presence of non-pathogenic E. coli K-12 and the probiotic L. rhamnosus GG. (a and b) Bacterial growth monitored by (a) OD₆₀₀ and (b) CFU mL^–1 for cultures of E. coli K-12 only, CFT073 only, and 1 : 1 K-12/CFT073 mixtures treated with 100 nM Amp or 100 nM (Glc)Ent–Amp 5/7/9 in the presence of 200 μM DP. (c and d) Bacterial growth monitored by (c) OD₆₀₀ and (d) CFU mL^–1 for cultures of E. coli K-12 only, UTI89 only, and 1 : 1 K-12/UTI89 mixtures treated with 100 nM Amp or 100 nM (Glc)Ent–Amp 5/7/9 in the presence of 200 μM DP. (e and f) Bacterial growth monitored by (e) OD₆₀₀ and (f) CFU mL^–1 for cultures of L. rhamnosus GG only, E. coli CFT073 only, and 1 : 1 L. rhamnosus GG/E. coli CFT073 mixtures treated with 1 μM Amp or 1 μM (Glc)Ent–Amp 5/7/9 in the presence of 200 μM DP. All mixed-E. coli antimicrobial assays were performed in 50% MHB medium and all mixed-species antimicrobial assays were conducted in 1 : 1 MRS/MHB medium (t = 19 h, T = 30 °C) (mean ± standard deviation, n = 3). An asterisk indicates OD₆₀₀ < 0.01 or no colony formation. The data for (Glc)Ent–Amx 6/8/10 are presented in Fig. S16.† .