Figure 2.

eIF5A depletion reveals increased pauses at proline, glycine and charged amino acids. (A) The correspondence between the ribosomal sites occupied by codons and sequencing reads, as measured by 5PSeq. (B) The differential ribosome pausing for all amino acids at –11, –14 and –17 nt from the selected codon. Significantly regulated differences (adjusted P-value < 0.01) in red. (C) The metagene that represents the 5PSeq intermediates for the indicated amino acids and positions. To facilitate the identification of codon-specific regulation and sample comparison, the total number of reads for each metagene (–80 to +20 window) was normalized.