Figure 3.

Multiple tripeptide motifs influence eIF5A-dependent translation. (A) Differential ribosome pausing for all tripeptide motifs. Axes represent the raw number of reads for the metagene of each motif. Significantly regulated differences (adjusted P-value < 0.0001) in red, computed using shrunken log₂ fold changes (see the Methods). (B) Hierarchical cluster depicting tripeptide motifs associated to eIF5A-dependent stall at –11nt. (C–H) The metagene that represents the 5PSeq intermediates for the selected tripeptide motifs. The total number of reads for each metagene (–80 to +20 window) was normalized.