Table 2. Thermodynamic parameters obtained from the fit of Equation (1) to the binding isotherms in Figure 7.
| [DNA] (mM) | [Hfq–CTR] (mM) | ΔHb (kJ mol−1) | K b (104 M−1) | n | ΔG (kJ mol−1) | ΔS (mol−1 K−1) |
|---|---|---|---|---|---|---|
| 2.6 | 0.3 | −23.9 ± 0.6 | 2.2 ± 0.1 | 0.51 ± 0.01 | −24.7 ± 0.2 | 3 ± 3 |
| 2.5 | 0.5 | −23.0 ± 0.5 | 1.7 ± 0.1 | 0.51 ± 0.01 | −24.1 ± 0.2 | 4 ± 2 |
| 1.6 | 0.2 | −24.3 ± 0.7 | 3.1 ± 0.3 | 0.81 ± 0.02 | −25.6 ± 0.3 | 4 ± 3 |
ΔHb, Kb, n, ΔG and ΔS denote the binding enthalpy, binding constant, stoichiometry, free energy and entropy changes, respectively.