Table 2. Thermal stability of AT11 and variant sequences with different bulges and linkers.
Sequence (5΄-3΄) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Name | Bulge | Linker | T m (°C) | |||||||||||||||||
AT11 | T | GG | T | GG | T | GG | TT | G | TT | G | T | GG | T | GG | T | GG | T | GG | T | 43 |
AT11-B1 | T | GG | T | GG | T | GG | TT | G | T | G | T | GG | T | GG | T | GG | T | GG | T | 45 |
AT11-B0 | T | GG | T | GG | T | GG | TT | G | G | T | GG | T | GG | T | GG | T | GG | T | 59 | |
AT11-L0 | T | GG | T | GG | T | GG | TT | G | TT | G | GG | T | GG | T | GG | T | GG | T | 51 | |
AT11-L2 | T | GG | T | GG | T | GG | TT | G | TT | G | TT | GG | T | GG | T | GG | T | GG | T | -a |
aUnable to detect fully-folded state at 20 °C