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. 2017 May 18;45(12):7285–7298. doi: 10.1093/nar/gkx454

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Primer extension analysis of uPSS-mediated transcripts of ribosomal protein genes rpsO (A) and rps17e (B), and thermosome-related genes cpn60 (C) and tap (D). Blue and magenta arrows indicate TSSs and PSSs, respectively. RBSs are shadowed by red box. Sequencing reads of the TSS in (+) library, both TSS and PSS in (−) library and PSS only in (5΄P) library are shown in the left panels. The y-axis scale represents the read count of a given site. Primer extension gels are shown in the right panels. The numbers 18 and 8 on the gel top indicate RNA isolated from R15 cultures grown at 18°C and 8°C, respectively.