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. 2017 May 18;45(12):7474–7486. doi: 10.1093/nar/gkx434

Figure 5.

Figure 5.

The control of mRNA levels is not dependent on riboswitch identity. (A and B) β-Galactosidase assays using translational BtuB–LacZ and transcriptional btuB–lacZ fusions (A) and transcriptional btuB–thiM–lacZ fusions (B). Enzymatic activities were measured in the absence or presence of 5 μM adenosylcobalamin (AdoCbl) or 500 μg/ml TPP. Values were normalized to enzymatic activity obtained without ligand. Schematics representing transcriptional constructs are shown on the right. The average values of three independent experiments with SDs are shown. (C) β-Galactosidase assays of the wild-type strain performed in the absence or presence of 5 μM AdoCbl or 25 μg/ml BCM. Values were normalized to enzymatic activity obtained for wild-type constructs without ligand. The average values of three independent experiments with SDs are shown.