Figure 4.

Exo-MuGENT used to rapidly assess the role of DNA binding sites for the nucleoid occlusion protein SlmA. (A) Exo-MuGENT was performed in the indicated strains using 100 ng of a selected product and 3000 ng each of five distinct unselected products (0.04/3 kb) that introduce point mutations into high-affinity SBSs. Results are shown as the frequency of strains with the indicated number of genome edits following one cycle of MuGENT. (B) Cell division accuracy histograms of the indicated strains. Inset values indicate division accuracy, which is defined as the total percentage of cells with a constriction site distant from mid-cell by <5%, and the total number of cells analyzed. (C) Phase contrast images of rare asymmetric cell division events observed in the Δ10×SBS strain background. Scale bar = 2 μm.