Skip to main content
. 2017 May 31;45(12):7527–7537. doi: 10.1093/nar/gkx496

Figure 5.

Figure 5.

Exo-MuGENT for rapid genetic dissection of diguanylate cyclases. (A) Distribution of genome edits in a population of cells following one cycle of Exo-MuGENT using single PCR mutant constructs (0.04/3 kb) that target 12 DGC genes for inactivation. (B) MASC-PCR gel for DGC genome edits in intermediate strains leading to generation of the Δ12 DGC mutant. (C) Intracellular c-di-GMP concentration of the indicated strains at low cell density (LCD) and high cell density (HCD). Data are from five independent biological replicates and shown as the mean ± SD. (D) Representative image of a swim assay of the indicated strains with quantification of the swim diameter from six biological replicates shown below as the mean ± SD. (E) Biofilm assay for the indicated strains. pMLH17 harbors an arabinose-inducible copy of the vpsR gene. (F) Swim assays of the indicated strains. Data are from at least eight independent biological replicates and shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and NS = not significant.