Table 1. Quantification of the kinetics of DNA compaction for wild-type BsSpo0J and mutants.
| Lag time (s) | Rate of DNA compaction (μm s−1) | ||||
|---|---|---|---|---|---|
| Protein | Mean | SD | Mean | SD | n |
| WT | 5.7 | 1.3 | 0.33 | 0.11 | 160 |
| E52R | 5.8 | 0.8 | 0.89 | 0.25 | 45 |
| E78R | 4.2 | 0.9 | 1.43 | 0.60 | 36 |
| F81A | 59.7 | 23.6 | 0.05 | 0.04 | 49 |
| G77S | 6.3 | 1.3 | 1.07 | 0.36 | 67 |
| G77S + R79A | 6.8 | 1.2 | 1.38 | 0.56 | 44 |
| I74A | 2.8 | 0.7 | 2.51 | 0.98 | 41 |
| M104A | 24.9 | 8.0 | 0.19 | 0.07 | 45 |
| N112S | 2.5 | 0.7 | 0.79 | 0.29 | 44 |
| P62A | 3.6 | 1.0 | 1.14 | 0.30 | 33 |
| Q140A | 11.1 | 6.2 | 0.05 | 0.02 | 66 |
| Q140R | 3.2 | 4.1 | 0.05 | 0.02 | 47 |
| Q61A | 36.8 | 13.5 | 0.12 | 0.06 | 53 |
| Q61R | 3.9 | 0.6 | 1.17 | 0.28 | 39 |
| R105A | 19.6 | 9.0 | 0.09 | 0.03 | 59 |
| V75E | 4.9 | 1.7 | 1.94 | 0.72 | 70 |
| Y72A | 3.9 | 1.1 | 2.02 | 0.65 | 38 |
Lag time was defined as the time between protein binding and the initiation of DNA compaction (Figure 3B and Supplementary Figure S8A). Rate of DNA compaction was estimated by linear fitting of the DNA length trajectory between maximum and minimum values (Supplementary Figure S8C). Distributions of lag time and rate of DNA compaction were fit to a Gaussian distribution (Supplementary Figure S8B and D). The total number of trajectories, n, was obtained from analysis for at least two replicates of experiments. SD = standard deviation.