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The effect of 4-O-Methylhonokiol (MH) on TGF-β1-induced Smads translocation in HaCaT cell. Nuclear translocation of Smad proteins was analyzed by separating cytoplasmic and nuclear fractions after treating HaCaT cells with TGF-β1 and/or MH for 1 h. Smad proteins were detected by Western blotting using antibodies against Smad2/3 and Smad4, respectively.
