Fig. 4.

The effect of 4-O-Methylhonokiol (MH) on TGF-β1-induced nuclear tranlocation of Sp1 inHaCaT cell. (A) HaCaT cells were treated with TGF-β1 (10 ng/mL) for the indicated times. Nuclear fractions were examined by 8% SDS-PAGE and analyzed with Western blotting using antibody against Sp1. (B) After pretreatment with MH (3, 30 and 300 nM) for 1 h, HaCaT cells were treated with TGF-β1 (10 ng/mL) for another 1 h. The nuclear tranlocation of Sp1 was analyzed by Western blotting. (C) HaCaT cells were incubated with MH (3, 30 and 300 nM) for 1 h, and the expression of Sp1 was analyzed. Blots were then probed with the corresponding antibodies after which they were stripped and reprobed with an antibody to PCNA to ensure equivalent loading and transfer.