Fig. 9.

Effect of 4-O-Methylhonokiol (MH) on the TGF-β1-induced NADPH oxidase NOX4 in HaCaT cells. (A) After serum starvation for 24 h, HaCaT cells were treated with TGF-β1 (10 ng/mL) for the indicated times. RNA extraction was carried out in RNase-free environment and the mRNA expression of NOX1, NOX2 and NOX4 were determined by RT-PCR. (B) After pretreated with MH (3, 30, 300 nM) for 1 h, HaCaT cells were incubated with TGF-β1 (10 ng/mL) for 30 nim. The mRNA expression of NOX1, NOX2 and NOX4 were determined by RT-PCR as described above. (C) HaCaT cells were treated with MH (3, 30, 300 nM) for 1 h, and the mRNA expression of NOX1, NOX2 and NOX4 were determined by RT-PCR as described above.