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Construction of the dG–APred-containing plasmid for cellular replication studies. (A) Duplexes used for constructing the plasmids for the replication study. Duplexes A, B and C are the inserts used for construction of the competitor, control, and dG–APred cross-link-bearing plasmids, respectively. ‘X’ represents uracil in the initial duplex and is converted to an abasic site prior to the cross-linking reaction. (B) Workflow for the preparation of the lesion-bearing plasmid.
