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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 24;507:192–198. doi: 10.1016/j.virol.2017.04.022

Fig. 3.

Fig. 3

DROSHA and DICER mRNA levels in HPV16 E6 and/or E7 expressing primary human foreskin keratinocytes. Three donor and passage matched HFK populations with stable expression of HPV16 E6 (E6), HPV16 E7 (E7), HPV16 E6/E7 (E6/E7) or an empty vector (C) were analyzed for (A) DROSHA and (B) DICER mRNA expression by RT-qPCR. Expression of 18S ribosomal RNA (18S) was utilized as a control and values were normalized to control vector transduced HFKs. Similar results were obtained with three additional HFK populations. Error bars show standard deviation. Unpaired, two-tailed t-tests with a 95% confidence level were performed to determine statistical significance. *, **, *** and **** indicate statistical significance at a P<0.05, P<0.01, P<0.001 and P<0.0001 respectively. “NS”: Not Statistically Significant. Please note that these HFK populations are distinct from those shown in Figure 4.