Skip to main content
. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Trends Genet. 2017 May 19;33(7):479–489. doi: 10.1016/j.tig.2017.04.006

Figure 3. DSBs Signal to Inhibit V(D)J Recombination.

Figure 3

A. RAG DSBs induced at Igκ loci in pre-B cells signal via ATM to transiently feedback inhibit further initiation of Igκ recombination. ATM signals transcriptional repression of RAG expression and transcriptional up-regulation of the SpiC transcriptional repressor. SpiC antagonizes Igκ recombination potential by displacing PU.1 from the Igκ intronic enhancer (iEκ) and by inhibiting pre-BCR signals, which activate Igκ transcription via PU.1/IRF4 binding to iEκ. B. DSBs induced in pre-B cells by genotoxic agents signal via distinct ATM-dependent pathways to repress transcription of Rag1 and Rag2.