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. 2017 May 17;45(11):6981–6992. doi: 10.1093/nar/gkx430

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Gated flow cytometry data for reporter constructs. (A) Analysis of flow cytometry data to determine the relationship between the gate radius and CV(%) for constructs containing yEGFP. Ten experiments were performed for each of the genomic constructs; this panel illustrates the variation in CV values for three of these constructs. Using a gate radius of 4000 (dashed vertical line in panel A), we obtained plots of CV(%; solid bars) versus mean fluorescence intensity values (hatched bars) for yEGFP (B) and for ymNeonGreen (C) constructs. The greater fluorescence intensity of ymNeonGreen enabled us to distinguish fully the fluorescence intensity distributions of P_TEF1(M3Un) and P_TEF1(G₁₄) from host cell autofluorescence, and to distinguish partially the distribution for P_DCD1(L0) (Supplementary Figure S3).