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Characterization of immortalized brown preadipocytes derived from BAT. (A) Schematic of the adipogenesis assay. (B) Cell morphology under microscope. (C) Oil Red O staining of differentiated cells 7 days after inducing adipogenesis. (D) qRT-PCR analysis of gene expression at indicated time points of adipogenesis. (E) qRT-PCR analysis of Ucp1 and Pparg gene expression in fully differentiated adipocytes (D7, day 7) that were treated with 100 nM CL-316 243 for up to 6 h.
