Table 3. Summary of restriction digest and DNA sequencing verification for 16, 21 and 31 kb assemblies.
| Assembly | RE digest | P(Dig correct) | Seq J1 | Seq J2 | Seq J3 | Seq J4 | Seq J5 | P(Seq correct) |
|---|---|---|---|---|---|---|---|---|
| 16kb TPA | 20/20 | 100% | 8/8 | 6/8 | 7/8 | 8/8 | 6/8 | 49% |
| 21kb TPA | 13/19 | 68% | 8/8 | 8/8 | 6/7 | 8/8 | 8/8 | 86% |
| 31kb TPA | 7/15 | 47% | 6/7 | 6/7 | 6/6 | 7/7 | 6/7 | 63% |
| GC TPA | 14/14 | 100% | 7/8 | 8/8 | 8/8 | 8/8 | - | 88% |
| 16kb GA | 11/13 | 85% | 7/7 | 0/8 | 6/8 | 7/8 | 8/8 | 0% |
| GC GA | 11/13 | 85% | 4/8 | 8/8 | 5/8 | 7/8 | - | 27% |
RE (Restriction Endonuclease) digest column shows the fraction of clones that were digested correctly. Seq J1-5 columns show the fraction of clones that were sequenced correctly at each junction. P (Dig correct) is the probability of a random clone having the correct digestion pattern, and P(Seq Correct) is the probability of a random digestion correct clone having all correct junctions as determined by DNA sequencing.