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. 2017 Feb 22;45(11):e95. doi: 10.1093/nar/gkx133

Figure 1.

Figure 1.

Exo-seq and RNaseH-seq enrich 5΄ and 3΄ mRNA ends respectively. (A) Schematic representation of our method for isolating 5΄ (Exo-seq) and 3΄ (RNaseH-seq) transcript-ends. (B) An Integrative Genome Viewer plot showing data coverage of the nirvana-2 (nrv2) gene with Exo-seq and RNaseH-seq on RNA extracted from Drosophila heads cultured at three different temperatures (18, 25 and 29°C), together with full-length RNA-seq at 25°C. (C) An Integrative Genome Viewer plot of Exo-seq and RNaseH-seq of the CG14298 gene showing multiple (and previously unknown) TSS (upper track) and PAS (lower track).

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