Figure 5.

BEAF-32 is active at the transcriptional response at 29°C. (A) Heatmap of differentially expressed genes between 18 and 29°C at wild-type or beaf32^−/− samples, or between wild-type and beaf32^−/− samples at 18 or 29°C. A gene was included in the heatmap if it had an adjusted P-value < 0.1 and log2 fold change >0.75 in at least one of the pairwise comparison. (B) The motif enriched in genes upregulated at 29°C in wild-type flies versus 18°C wild-type (left), compared with the enriched motif in genes upregulated at 29°C wild-type versus 29°C beaf32^−/− flies (middle) and the enriched motif in genes upregulated at 29°C beaf32^−/− flies versus beaf32^−/− at 18°C (right). (C) Log2 Fold Change at 18 and 29°C between the wild-type and beaf32^−/− samples of the genes upregulated at 29°C wild-type versus 29°C beaf32^−/− and harbor the BEAF-32 binding motif at their promoter. Arrow represents the location of most of those genes on the heatmap. Asterisk represents P-value < 0.05 between the distributions of the fold change using a t-test. Error bar represents the standard deviation.