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. 2017 May 3;45(11):6894–6910. doi: 10.1093/nar/gkx307

Figure 8.

Figure 8.

Regulation of ACOT7 mRNA and protein levels in a WIG1-dependent manner following irradiation. (A) MCF7 cells were harvested after exposure to 2, 4 or 6 Gy of ionizing radiation (IR) and subjected to immunoblotting for detection of p53, WIG1 and ACOT7. (B) Cells were transfected with Con Si or WIG1 Si, exposed to 6 Gy of IR, incubated for 24 h and then harvested for immunoblot and RT-PCR analyses. (C) After transfection with anti-miR-9, MCF7 cells were exposed to IR and then harvested for immunoblot and RT-PCR analyses. Actin was used as an internal control (A–C). Each bar in the graphs represents the mean ± SD compared to control cells from three independent experiments (B and C). *P < 0.05; **P < 0.01; #P > 0.05. (D) Proposed model for novel dual role of WIG1: miRNA-dependent mRNA decay and miRNA-independent translational suppression. RNA-binding protein WIG1 is a novel dual repressor of gene silencing. WIG1 regulates target mRNA stability in an miRNA-dependent manner and suppresses translational initiation in an miRNA-independent manner.