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. ACBD4iso2/VAPB co-expression promotes PO-ER association. COS-7 cells were transfected with (A) Myc-VAPB alone (immunolabelled using αPEX14, a peroxisomal marker), (B) Myc-VAPB co-expressed with GFP-ACBD4iso2, (C) Myc-VAPB co-expressed with GFP-ACBD4iso2 showing mitochondrial mistargeting. (D) Co-localization of GFP-ACBD4iso2 with Tom20 (mitochondrial marker). Arrows highlight PO-ER association. Bars, 20 µm (overview), 5 µm (cut outs).
