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. 2017 May 8;45(11):6644–6655. doi: 10.1093/nar/gkx357

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — In vitro analysis of Muta1 double strand cleavage. (A) The 188-bp DNA substrate is 5΄ radiolabeled and contains full length Muta1 L-TIR (145 bp) and a 43 bp flanking segment. The possible reaction outcomes of the three DNA cleavage pathways in Figure 1 are shown. (B) Reaction products on a native polyacrylamide showing 43 and 145 bp Muta1 L-TIR products (lanes 5–8). Diagram on right indicates the predicted structure and size of each band. (C) Reaction products on a denaturing polyacrylamide gel showing the 145 and 86 nt bands (lanes 4–8).