Figure 8.

Analysis of the impact of Muta1 subterminal repeats and terminal motif on transposition in yeast. (A) Muta1NA1 contains the 10-bp terminal motif at both ends; three and seven copies of the subterminal repeats on the left and right ends, respectively, and 315 bp of internal DNA sequence. (B) Excision frequencies of Muta1NA1 derivative elements generated by altering the copy number of the two motifs at both ends of Muta1NA1. TIR composition of each element is shown at the bottom (see text for details). Six replicates were used for each. (C) Excision frequencies of mutant versions of Muta1NA1. Mutations were introduced at both ends of the terminal motif of Muta1NA1, as indicated by underlined letters. Six replicates were used for each.