Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 2;45(11):6881–6893. doi: 10.1093/nar/gkx306

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Aggregation of Sup35 in response to H₂O₂ stress. (A) Protein aggregates were isolated from the wild-type strain grown in the presence of 0.5 mM (+) or 1 mM (++) H₂O₂ for 6 h and analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. T, total cell extracts; S, soluble fraction; I, insoluble aggregate fraction. Four-times as much of the insoluble fraction was loaded relative to the total or soluble fractions to aid visualization. (B) The same protein samples as for panel A were analyzed by western blotting using antibodies that recognize Sup35 and Pgk1 as a loading control. (C) Quantification of soluble and insoluble Sup35 (percentage of total) as shown in panel B from triplicate experiments. Error bars denote SD. (D) Sup35-GFP or GFP was visualized in the wild-type strain exposed to 1 mM H₂O₂ for 6 h. Examples of cells containing visible Sup35-GFP puncta are shown. The percentage of cells containing visible puncta was quantified from three independent biological repeat experiments ± SD.