Figure 5.

Cofactor steady-state kinetics are affected by the mutation of ATXR5 PHD domain. Michaelis–Menten (M–M) plot of the initial velocity versus nucleosome concentration (A) and its Lineweaver–Burk (LB) double reciprocal plot (B) for full-length wild-type ATXR5, L39W mutant and ATXR5 ΔPHD. The kinetics for the cofactor were performed using 8 μM of recombinant nucleosome. The M-M plot of the initial velocity versus cofactor concentration (C) and its corresponding LB plot (D) for the constructs used in C. (E) Summary of the results obtained from the M-M plots for wild-type ATXR5, L39W mutant and ATXR5 ΔPHD.