Figure 1.

MYB15 interacts with MPK6 in vitro and in planta. (A) Pull-down assay demonstrating the specificity of the interaction of MPK with MYB15. Shown is chemiluminescence detection of (His)₆ on blots of SDS-polyacrylamide gels loaded with (left to right panels) input His-MPKs, GST and GST-MYB15 pull-down proteins. (B) LUC complementation imaging assay to detect the interaction of MPK6 with MYB15 in Nicotiana benthamiana leaves. Shown in the upper panel are images and luminescence of N. benthamiana leaves co-infiltrated with Agrobacterium strains containing the indicated combinations of NLuc- and CLuc-fusion constructs. The SGT1a-NLuc/CLuc-RAR1 combination was used as a positive control. Vec refers to NLuc and CLuc empty vectors. Leaves were photographed at 3 days after infiltration. Shown in the lower panel is the quantification of LUC activity in these leaves. The luminescence intensities are expressed relative to that of leaves infiltrated with the NLuc Vec/CLuc Vec combination. Data are presented as the mean ± SD of three independent experiments. P < 0.001 (***) indicate statistically significant changes.