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. 2017 May 16;45(11):6613–6627. doi: 10.1093/nar/gkx417

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Identification of MPK6-phosphorylated peptides in GST-MYB15N using TiO₂ chromatography and MALDI-TOF mass spectrometry. (A and B) Shown are the peptide mass fingerprint of unphosphorylated (A) or MPK6-phosphorylated (B) GST-MYB15 N-terminal fragment (amino acids 1–172) after trypsin digestion and without TiO₂ purification. A database search of mass data identified GST and MYB15N in the sample with 62 and 38% coverage, respectively. (C and D) Shown are the mass fingerprints of phosphopeptides recovered after TiO₂ enrichment from tryptic digests of unphosphorylated (C) or MPK6-phosphorylated (D) GST-MYB15N. One phosphopeptide peak (m/z 2482.9) originating from SESELADSSNPSGESLFSTSPSTS was detected from phosphorylated GST-MYB15N (D) but not from unphosphorylated GST-MYB15N (C).