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MYB15 is phosphorylated by cold-activated plant MPK6. Leaves of 3-week-old wild-type (WT) and mpk6 plants were subjected to cold (4°C) treatments for the indicated times to induce MPK activity. Shown are autoradiographs of SDS-polyacrylamide gels containing embedded MBP, GST-MYB15 and GST, depicting signals from an in-gel kinase assay on resolved proteins from extracts of treated leaves. The expected positions of MPK4 and MPK6 on the gels are indicated.
