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Binding affinity of MYB15 to the CBF3 promoter fragment is reduced by MPK6-induced phosphorylation. Shown are the results of an EMSA performed using a 32P-labeled CBF3 promoter fragment (−984 to −785 bp region) as a probe. Equal amounts of purified Escherichia coli-expressed GST, GST-MYB15 (A) and GST-MYB15S168A (B) were incubated in the absence or presence of purified His-MPK6 at 30°C for 30 min before adding the probe.
