Figure 7.
Transcriptional repression activity of MYB15 is enhanced by a mutation in its phosphorylation site. (A) Schematic representation of reporter and effector constructs used in the transient expression assay. The effector construct consisted of the CaMV35S promoter fused to the full-length MYB15 and MYB15S168A ORFs. The CBF3 promoter (−2000 to −1 bp region) was fused to the GUS reporter gene containing a minimal CaMV35S promoter to generate the reporter construct. (B) Transient assay of CBF3 expression in Arabidopsis protoplasts. The GUS//LUC activities of Arabidopsis protoplasts that were co-transfected with the CBF3: GUS reporter plasmid, a CaMV35S: LUC internal control plasmid and the indicated effector plasmids were measured. Shown are relative GUS/LUC activities, which were calculated as a percentage of the GUS/LUC activities of samples transformed with the reporter construct plus empty effector vector. Data are presented as the mean ± SD of three independent experiments. P < 0.001 (***) indicate statistically significant changes.