Figure 8.
Mutation of the MYB15 phosphorylation site increases its transcriptional repression of CBF genes in cold-stressed transgenic plants. CBF expression was measured in transgenic plants expressing empty vector (EV), MYB15 and MYB15S168A under cold stress. Quantitative RT-PCR analysis of CBF genes was performed using total RNA isolated from seedlings of the indicated lines subjected to cold (4°C) treatment for the indicated times. The cDNA was synthesized from 5 μg of total RNA and used as a template for RT-PCR. Data are presented as the mean ± SD of three independent experiments. P < 0.05 (*), P < 0.01 (**) and P < 0.001 (***) indicate statistically significant changes.