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. 2017 Apr 5;45(11):6793–6804. doi: 10.1093/nar/gkx239

Figure 4.

Figure 4.

GLD4 regulates glucose metabolism. (A) U87MG cells were transduced with shNS, or shRNAs targeting GLD4 or CPEB1 and measured for glucose uptake (mean ± SEM) (n = 4) after 72 h. (B) U87MG cells were transduced with lentivirus expressing empty vector (control) or a GLUT1 expressing vector and 24 h later the same cells were transfected with siNT or siGLD4. After 48 h, cells were measured for glucose uptake (mean ± SEM) (n = 3). (C) U87MG cells were transfected with siNT or siGLD4 and measured for lactate levels (mean ± SEM) (n = 4). (D) U87MG cells were transduced with lentivirus expressing empty vector (control) or a GLUT1 expressing vector and 24 h later the same cells were treated with siNT or GLD4. After 48 h, cells were measured for lactate levels (mean ± SEM) (n = 4). * indicates P < 0.05, ** indicates P < 0.01 and *** indicates P < 0.001 by two-tailed Student's t-test.