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. 2017 Apr 5;45(11):6793–6804. doi: 10.1093/nar/gkx239

Figure 5.

Figure 5.

GLD4 regulates glucose deprivation-induced GLUT1 expression. (A) GLUT1 protein levels upon glucose deprivation (Glu–) in control (siNT) or GLD4 knockdown U87MG cells (siGLD4). 0 h rindicates pre-deprivation and Glu+ indicates a non-deprivation control. Glu– indicates glucose deprivation for 24 h. EIF4E and GAPDH were used as loading controls. (B) Relative GLUT1 protein levels were quantified compared to 0 h of the control cells (mean ± SEM) (n = 6). (C) The same set of cells as (B) was subjected to RT-qPCR to test GLUT1 mRNA levels. (D) Relative GLUT1 protein synthesis is calculated by dividing numbers from (B) with those from (C). * indicates P < 0.05, ** indicates P < 0.01 and *** indicates P < 0.001 by two-tailed Student's t-test.