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. 2017 Mar 16;45(11):e101. doi: 10.1093/nar/gkx181

Figure 1.

Figure 1.

Human CRISPR library characteristics. (A) Potency score distribution of the human (Hs) exon library. The potency score cut-off was 0.2. (B) Guide score distribution of the human exon library. Library sgRNAs were ranked separately for their guide score for coding regions (guide_c) and guide score for non-coding regions (guide_nc). The cut-off for guide_c score was 50 and for guide_nc was 10. (C) Distribution of sgRNA counts for all genes in the human exon library. (D) Potency score distribution of the human domain library (no cut-off was imposed for this library). (E) Guide score distribution of the human domain library. Library sgRNAs were ranked separately for their guide_c scores (cut-off at 50) and guide_nc scores (cut-off at 10). (F) Distribution of sgRNA counts for all genes in the human domain library. (G) Distribution of sgRNA counts for all genes in the combined human exon and domain library. (H) Distribution of off-target site counts for sgRNAs in the combined human exon and domain library. C1, C2 and C3 represent off-target sites in coding regions with up to one, two or three mis-matches, respectively.