Figure 3.

Location of sgRNAs from various CRISPR KO libraries in the human RAF1 and BRD4 genes. The positions of sgRNAs from our library and other published genome-wide libraries (8,12,16,18–20) in the gene are indicated as short lines. For our library, sgRNAs from the exon library are colored green and those from the domain library are colored blue. An * above the sgRNA indicates the target sequence spans an intron–exon junction. Identical sgRNA sequences from different libraries are connected by a vertical red line.