Figure 1:

Schematic of viral disease development (VDD) discovery, refinement, validation and application datasets. The VDD discovery dataset was identified from published microarray viral challenge studies that included five RNA virus species. In house (MGL) IHNv challenge microarray studies across four salmon species were used to refine the VDD panel. Analytical validations of the qRT-PCR assays developed to 45 biomarkers within the VDD panel was performed using independent in-house studies that tested discrimination abilities of the proposed VDD between latent and disease-associated viral infections across tissues, salmon and viral species, as well as differentiation of fish undergoing viral, bacterial, and parasitic diseases. The VDD panel was then applied to wild migrating Sockeye salmon smolts to discern whether wild fish infected with IHNv could be identified in a VDD state.