Figure 1.

Targeted recruitment of GFP to major satellites. (A) Schematic outline of dCas9-mediated effector recruitment. GBP-dCas9-mRFP is guided to a desired locus by a sgRNA and interacts with a GFP-coupled epigenetic effector via GBP. Subsequently, the effector modifies the underlying DNA. (B-C) Representative confocal images of ESCs, co-transfected with GBP-dCas9-mRFP and major satellite specific sgRNA. GBP-dCas9-mRFP specifically localizes at CCs and recruits GFP, when it is additionally co-transfected (C). Line plots represent the signal intensity of the different channels along the indicated chromocenter (solid white line). White dashed lines indicate the nuclear border. Scale bar: 10 µm.