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. 2017 May 17;16(12):1145–1152. doi: 10.1080/15384101.2017.1320628

Figure 2.

Figure 2.

Sister centromeres come under tension but fail to disjoin upon co-expression of non-degradable versions of Sgo1 and Mps1 in meiosis II. Control (z26823) and PEST-sgo1-mD PEST-mps1-mD (z26825) cells containing the CDC20-mAR synchronisation system were released from the metaphase I arrest with CuSO4 (10 μM, t = 0). Estradiol (10 μM) was added at t = 15 min to induce PEST-sgo1-mD and PEST-mps1-mD.43 Nuclei containing histone H2B tagged with the RFP mCherry and the sister centromeres of one chromosome V copy labeled with the CEN5-tetO/TetR-GFP system (CEN5-GFP)19 were imaged every 10 min. Top, representative time-lapse series. The width of one frame is 5 μm. Middle, percentages of cells with one division (2 nuclei), 2 divisions (more than 2 nuclei), split GFP signals (2 GFP-dots in the same nucleus), and segregated GFP signals (GFP signals in separate nuclei). Bottom right, PEST-sgo1-mD PEST-mps1-mD cells that fail to undergo the meiosis II division are plotted separately.