Figure 3.

A targeted Y2H analysis using the proteins identified in the liquid chromatography-mass spectrometry (LC-MS). T975–1053 and one of the proteins identified in the LC-MS (see Table S1 for their information) were co-expressed as GAL4 DNA-binding domain (BD)-fused protein and GAL4 activation domain (AD)-fused protein, respectively, in Y2HGold Yeast Strain. For –, the BD alone was co-expressed with an AD-fused protein. The transformed cells were grown on the selection medium DDO/X/A, which contains X-α-gal and the Aureobasidin A antibiotic. More than 30 individual colonies obtained with the transformation were used for the analysis (see Table 1 for the exact numbers of the colonies used), and the representative results are presented.