Figure 2.

Effects of Palbociclib on cell death and cell cycle in CRC cell lines. RKO colon cancer cells were treated at the indicated time points with palbociclib (10 μM) under normoxic (A and C) or hypoxic (B and D) conditions. Representative curves are shown in the upper panels (A and B). Bar diagrams (C and D) represent Sub-G1 distribution within the cell cycle profiles. Data are shown as Mean ± SD (n = 3, Student's t-test, 2-tailed, “*” represents P < 0.05, “**” represents P < 0.01 (E) Immunoblotting shows cleavage of apoptosis-related proteins with or without palbociclib under either normoxia (Left panels) or hypoxia (Right panels). Cell death and cell cycle alterations are shown using flow cytometry. Cell death after treatment of 48 hours under hypoxia was significant. Thus there were not enough cells to support cell cycle analysis (B).