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. 2017 May 31;16(12):1210–1219. doi: 10.1080/15384101.2017.1325042

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Figure 3. — Chromosomal abnormalities are detected in B56γ- MEFs. (A-D) Immunohistochemical analysis with antibodies specific for Histone H3 pSER10 (pH3, green), DAPI (blue) and α-Tubulin (red), showing lagging or misaligned chromosomes (shown with yellow arrows) in the B56γ- MEFs treated with nocodazole. Representative images were obtained using a 100X objective. Chromosomal lagging was recorded at approximately 2% per 1000 total cells in nocodazole treated B56γ- MEFs. Data are representative of 3 independent experiments, using 3 B56γ- and B56γ+ MEF samples. E) Time lapse microscopy live images captured at the indicated time points from NEBD of nocodazole treated B56γ- MEFs. Of the 21 B56γ- MEFs, 13 displayed abnormal chromosomal segregation (shown with yellow arrows).