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Endogenous expression of B56 subunits in MEFs. A) RT-PCR was used to detect native B56α, B56β, B56γ, B56δ and B56ε mRNA in wild type MEFs, -RT = no transcriptase. (B - F) Immunohistochemical analysis using antibodies to B56α, B56β, B56γ, B56ε (green) and B56δ (red) with DAPI (blue) in wild type MEFs. Representative immunofluorescence images from MEFs at E14.5. Representative images from 3 independent experiments were obtained using a 60X objective.
