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Nocodazole treatment of B56γ- MEFs depletes BubR1 and Cyclin B. Western blot analyses were performed on protein extracts from synchronized wild type (+/+) and B56γ- (−/−) MEFs. Less BubR1 is present in untreated B56γ- MEFs than in wild type MEFs. BubR1 and Cyclin B1 were not detected in the nocodazole treated B56γ- MEFs. No change was observed in the expression of the catalytic subunit of PP2A. Data are representative of 3 experiments, using 3 B56γ- and B56γ+ MEF extracts.
