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. 2017 May 31;16(12):1210–1219. doi: 10.1080/15384101.2017.1325042

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Figure 8. — Immunohistochemical analysis of BubR1 and Cyclin B1 during mitosis. BubR1 (red, A-H) or Cyclin B1 (red, I-P), Histone H3 pSer10 (pH3, green) and DAPI (blue) were detected by immunohistochemistry. Following 18 hour nocodazole (NOC), BubR1 expression was found to be absent throughout prophase in B56γ- (−/−) MEFs (E-H) but present in the wild type (+/+) MEFs (A-D). Less Cyclin B1 was observed in the nocodazole treated B56γ- MEFs (M, N) at early prophase compared with wild type MEFs (I, J). By late prophase, Cyclin B1 was not detectable (O, P) but present in the wild type MEFs (K, L). All images were captured at 60X magnification. Data are representative of 3 independent experiments from approximately 50 different cells from each genotype.