Figure 1.

Nup82 is a nucleoporin that is unique to angiosperms and is localized on the nuclear envelope. (A) The phylogenetic tree of Nup82 (yellow box) and Nup136 (purple box) proteins from representative angiosperms (Amborella trichopoda), monocots (Oryza sativa and Zea mays), eudicots (Nelumbo nucifera), and dicots (Glycine max, Populus trichocarpa and Arabidopsis thaliana). The aligned amino acid sequences were assembled into a phylogenetic tree using the boot-strapped neighbor-joining algorithm⁴⁸ in MEGA 6.06 with 1000 trials (http://www.megasoftware.net/). Bootstrap values are indicated as percentages of 1000 trials at their respective nodes. Labels at the branch tips indicate NCBI Reference Sequence Numbers or gene names. (B) Confocal fluorescence images of root tip cells from transgenic Arabidopsis stably expressing Nup82-GFP. (C) Confocal fluorescence images of protoplasts of Arabidopsis cultured cells transiently expressing Nup82-GFP. (D) Bimolecular fluorescence complementation (BiFC) assays in tobacco leaf epidermal cells. Histone-RFP was coexpressed as a marker for visualizing nuclei in the transformed cells. nYFP, N-terminal half of YFP. cYFP, C-terminal half of YFP.