Figure 3.

Defense-related genes are downregulated in the nup82 nup136 double mutant. (A) An enrichment analysis of Gene Ontology (GO) terms in the biologic processes category. REViGO amalgamated GO terms associated with the downregulated genes in the nup136 nup82 double mutant. (B) The top 10 genes in the group of defense response, which were downregulated in the nup82 nup136 double mutant. Bacterial response indicates whether gene expression is induced by bacterial infection, according to a public microarray analysis (AtGeneExpress). fold, fold change in gene expression between Control P.s. at 24 h vs Nonhost P.s. at 24 h. (C) Quantitative RT-PCR of defense-related genes in 14-day-old seedlings of the wild-type (WT), nup82–1, nup136–2, and nup82–1 nup136–2 plants. Data represent mean values of 3 independent experiments with standard deviations.