Figure 4.

Nup136 and Nup82 are involved in benzothiadiazole (BTH)-induced resistance against Pseudomonas syringae pv tomato virulent strain DC3000 (Pto DC3000). (A and B) Bacterial growth at 2 d after inoculation with Pto DC3000 (OD600 = 0.0001) in the leaves of nup136–2, nup82–1, and nup136–2 nup82–1 mutants that were pretreated without (A) or with (B) 150 µM BTH for 1 day. *, p < 0.05; **, p < 0.01 compared with the wild-type plants. Data represent mean values of 2 independent experiments (n = 16). Error bars indicate standard errors (Student's t test, *p < 0.05, **p < 0.01). (C) Quantitative RT-PCR of defense-related genes in 14-day-old seedling of WT and nup82–1 nup136–2 with (+BTH) and without benzothiazole (-BTH) treatment. Data represent mean values of 3 independent experiments with standard deviations.